SEM elisa kit product blog
Tags: ELISA Kit; Nitrofurazone; SEM elisa kit; SEM;
The SEM n/a (Catalog #MBS281044) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase.To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Nitrofural (INN, trade name Furacin) is a bactericidal compound used as an antibiotic most commonly in the form of ointments. Its use in medicine has become less frequent as safer and more effective products have become available, and it has been discontinued in the US. The substance is pale yellow and crystalline. Other names include nitrofurazone and furacilin.
Principle of the Assay: This assay is based on the competitive enzyme immunoassay for the detection of SEM in the sample. The coupling antigens are pre-coated on the micro-well stripes. The SEM in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-SEM antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the SEM in it. This value is compared to the standard curve and the SEM concentration is subsequently obtained.
Samples: Tissue, aquatic, honey, etc.
Assay Type: Competitive ELISA. Sample Volume: 10-200 uL
Precision: Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength: 450 nm. Nitrofurazone (SEM) ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.