SELE elisa kit product blog
Tags: ELISA Kit; Human ELISA Kit; E-Selectin; SELE; SELE elisa kit;
The Human SELE sele (Catalog #MBS494770) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS494770 ELISA Kit recognizes Human SELE.The SELE sele product has the following accession number(s) (GI #187960042) (NCBI Accession #NP_000441.2). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the E-Selectin ELISA with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Intended Uses: For detection of ErbB-2 in human serum, plasma, body fluids, tissue lysates or cell culture supernates. For research use only.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-ErbB-2 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-ErbB-2 polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the ErbB-2 amount of sample captu red in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of ErbB-2 can be calculated
Background/Introduction: ErbB-2 also known as HER2 (Human Epidermal Growth Factor Receptor 2), Neu, CD340 or p185, is a 185-kD glycoprotein with tyrosine kinase activity, and it is a member of the epidermal growth factor receptor (EGFR/ErbB) family. ErbB-2 is encoded by ERBB2, a known proto-oncogene located at the long arm of human chromosome 17(17q21-q22). ErbB-2 was named for its similarity to ERBB (avian erythroblastosis oncogene B). The ErbB family is composed of four plasma membrane-bound receptor tyrosine kinases. Unlike the other family members, ErbB-2 is considered to be an orphan receptor as it has no known ligand. Overexpression of ERBB2 and ERBB3 has been implicated in the neoplastic transformation of prostate cancer.
Samples: Human Serum, Plasma, Body Fluids, Tissue Lysates Or Cell Culture Supernates
Assay Type: Sandwich
Detection Range: 62.5pg/ml-4000pg/ml
Sensitivity: <10pg/ml. E-Selectin ELISA or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. The following patways have been known to be associated with this gene. Brain, Connective Tissue, Heart, Liver, Lung, Muscle, Placenta, Testis, Uterus, Vascular tissues are correlated with this protein. SELE also interacts with the following gene(s): ICAM1, JUN, NFKB1, RELA, TNF. Anemia, Atherosclerosis, Cardiovascular Diseases, Heart Diseases, Hypertension, Inflammation, Lung Diseases, Myocardial Infarction, Myocardial Ischemia, Necrosis are some of the diseases may be linked to E-Selectin ELISA.