|The Human SELE sele (Catalog #MBS355367) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS355367 ELISA Kit recognizes Human (General) SELE. MyBioSource\'s E-Selectin can be used in a range of immunoassay formats including, but not limited to, Quantitative sELISA (EIA).
For quantitative detection of E-Selectin in serum, plasma, body fluids, tissue lysates or cell culture supernates. Researchers should empirically determine the suitability of the SELE sele for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The SELE sele product has the following accession number(s) (GI #187960042) (NCBI Accession #NP_000441.2) (Uniprot Accession #P16581). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual
for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the E-Selectin Human ELISA Kit with the following immunoassay(s):
Typical Standard Curve/Testing Data
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- E-Selectin polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- E-Selectin polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the E-Selectin amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of E-Selectin can be calculated.
Background/Introduction: E-selectin, also known as CD62 antigen-like family member E (CD62E), endothelial-leukocyte adhesion molecule 1 (ELAM-1), or leukocyte-endothelial cell adhesion molecule 2 (LECAM2), is a cell adhesion molecule expressed only on endothelial cells activated by cytokines. The ELAM gene is present in single copy in the human genome and contains 14 exons spanning about 13 kb of DNA. E-selectin mediates the adhesion of tumor cells to endothelial cells, by binding to E-selectin ligands expressed by neutrophils, monocytes, eosinophils, memory-effector T-like lymphocytes, natural killer cells or cancer cells.
Samples: Serum, plasma, body fluids, tissue lysates or cell culture supernates
Assay Type: Quantitative Sandwich
Detection Range: 62.5 pg/ml-4000 pg/ml
Sensitivity: < 4 pg/ml. E-Selectin Human ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.