SDC3 elisa kit product blog
Tags: ELISA Kit; Mouse ELISA Kit; syndecan 3; SDC3 elisa kit; SDC3;
The Mouse SDC3 sdc3 (Catalog #MBS8291432) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS8291432 ELISA Kit recognizes Mouse SDC3.The SDC3 sdc3 product has the following accession number(s) (GI #42476310) (NCBI Accession #NP_035650.2) (Uniprot Accession #Q64519). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Mouse Syndecan 3 ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: The MyBioSource Mouse PCSK9 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse PCSK9 in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Mouse PCSK9 coated on a 96-well plate. Standards and samples are pipetted into the wells and PCSK9 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse PCSK9 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of PCSK9 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm
Background/Introduction: Proprotein convertase subtilisin/kexin type 9, also known as PCSK9, is an enzyme that in humans is encoded by the PCSK9 gene. This gene encodes a proprotein convertase belonging to the proteinase K subfamily of the secretory subtilase family. By genomic sequence analysis, the PCSK9 gene was mapped to chromosome 1p32. This protein plays a major regulatory role in cholesterol homeostasis. PCSK9 binds to the epidermal growth factor-like repeat A (EGF-A) domain of the low-density lipoprotein receptor (LDLR), inducing LDLR degradation. Reduced LDLR levels result in decreased metabolism of low-density lipoproteins (LDL), which could lead to hypercholesterolemia. PCSK9 may also have a role in the differentiation of cortical neurons.
Samples: Cell Culture Supernatants, Serum, Plasma
Sensitivity: 10 pg/ml. Mouse Syndecan 3 ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.