SCNN1B elisa kit product blog
Tags: ELISA Kit; Rabbit ELISA Kit; Amiloride-sensitive sodium channel subunit beta (SCNN1B); SCNN1B; SCNN1B elisa kit;
The Rabbit SCNN1B scnn1b (Catalog #MBS7240480) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS7240480 ELISA Kit recognizes Rabbit SCNN1B.The SCNN1B scnn1b product has the following accession number(s) (GI #124301196) (NCBI Accession #NP_000327.2). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Rabbit Amiloride-sensitive sodium channel subunit beta (SCNN1B) ELISA Kit with the following immunoassay(s):
Typical Standard Curve/Testing Data
Intended Uses: This SCNN1B ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rabbit SCNN1B. This ELISA kit for research use only!
Principle of the Assay: SCNN1B ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-SCNN1B antibody and an SCNN1B-HRP conjugate. The assay sample and buffer are incubated together with SCNN1B-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SCNN1B concentration since SCNN1B from samples and SCNN1B-HRP conjugate compete for the anti-SCNN1B antibody binding site. Since the number of sites is limited, as more sites are occupied by SCNN1B from the sample, fewer sites are left to bind SCNN1B-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SCNN1B concentration in each sample is interpolated from this standard curve.
Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type: Quantitative Competitive
Sensitivity: 1.0 pg/mL. Rabbit Amiloride-sensitive sodium channel subunit beta (SCNN1B) ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.