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S100B elisa kit product blog

Posted on 2016-06-18 16:17:01 by mybiosource_staff
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Tags: ELISA Kit; Sheep ELISA Kit; S100B elisa kit; S100B;
The Sheep S100B s100b (Catalog #MBS2504395) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS2504395 ELISA Kit recognizes Sheep (Ovine, General) S100B.

The S100B s100b product has the following accession number(s) (GI #49457242) (NCBI Accession #CAG46920.1) (Uniprot Accession #P04271). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

(Or you can also download the PDF Manual for complete product instructions).

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Sheep S100B concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to S100B. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for S100B and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain S100B, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of S100B. You can calculate the concentration of S100B in the samples by comparing the OD of the samples to the standard curve.

S100B s100b

Samples: Serum, plasma and other biological fluids
Assay Type: Quantitative Sandwich
Detection Range: 78.125-5000pg/mL
Sensitivity: 46.875pg/mL. Sheep S100B (S100 Calcium Binding Protein B) ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. S100B also interacts with the following gene(s): AHNAK, ANXA6, APP, GFAP, IQGAP1, S100A1, STK38, TP53. The following patways have been known to be associated with this gene. Adipose Tissue, Blood, Brain, Embryonic Tissue, Eye, Heart, Lung, Nerve, Skin, Spinal Cord tissues are correlated with this protein. Brain Diseases, Brain Injuries, Brain Ischemia, Cardiovascular Diseases, Disease Models, Animal, Heart Diseases, Hemorrhage, Inflammation, Necrosis, Nervous System Diseases are some of the diseases may be linked to Sheep S100B (S100 Calcium Binding Protein B) ELISA Kit.
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