S100a8 elisa kit product blog
Tags: ELISA Kit; Mouse ELISA Kit; S100A8 elisa kit; S100A8; S100A8 PicoKine;
The Mouse S100a8 s100a8 (Catalog #MBS177630) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS177630 ELISA Kit recognizes Mouse S100a8.The S100a8 s100a8 product has the following accession number(s) (GI #7305453) (NCBI Accession #NP_038678.1) (Uniprot Accession #P27005). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Mouse S100A8 PicoKine ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only) (Mouse S100A8 PicoKine ELISA Kit standard curve )
Principle of the Assay: The mouse S100A8 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for S100A8 has been precoated onto 96-well plates. Standards (Expression system for standard: E.coli, Immunogen sequence: P2-E89) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for S100A8 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse S100A8 amount of sample captured in plate.
Background: S100 calcium-binding protein A8 (S100A8), also known as calgranulin A, is a protein that in humans is encoded by the S100A8 gene. It is mapped to chromosome 1q21.3 based on an alignment of the S100A8 sequence with the genomic sequence. The proteins S100A8 and S100A9 form a heterodimer called calprotectin which is a major calcium- and zinc-binding protein in the cytosol of neutrophils, monocytes, and keratinocytes. This gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. And S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and as a cytokine. Altered expression of this protein is associated with the disease cystic fibrosis.
Samples: Cell culture supernates, serum and plasma (heparin, EDTA).
Assay Type: Sandwich
Detection Range: 31.2pg/ml-2000pg/ml
Sensitivity: < 10pg/ml.