Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource  rightarrow Assay Kit Blog  rightarrow S100A12 elisa kit  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Blogs moreseparator
separator
 • sCD86 elisa kit blog
 • ARF1 elisa kit blog
 • CFH elisa kit blog
 • MRP elisa kit blog
 • SYT13 elisa kit blog
 • PSMA6 elisa kit blog
 • GDF6 elisa kit blog
 • ACTC1 elisa kit blog
 • CLCF1 elisa kit blog
 • FcgammaBP elisa kit blog
 • alpha1-AT elisa kit blog
 • SVCAM-1 elisa kit blog
 • SCD27 elisa kit blog
 • CRISP3 elisa kit blog
 • ALOX12 elisa kit blog
 • PR3 Ab elisa kit blog
 • OR2T6 elisa kit blog
 • EPX elisa kit blog
 • SLC8A1 elisa kit blog
 • CER elisa kit blog
separator
Blog Categories separator
separator
 • Assay Kit Blogs
 • Biochemical Blogs
 • cDNA Clone Blogs
 • Monoclonal Antibody Blogs
 • Polyclonal Antibody Blogs
 • Rec/Purified Protein Blogs
 • Popular Product Blogs
separator
Our Best Sellers moreseparator
separator
 • M-CSF Recombinant Protein
 • Progesterone (Pg) ELISA Kit
 • Alkaline Sphingomyelinase (Alk-Smase) Recombinant Protein
 • A Disintegrin And Metalloprotease 22 (ADAM22) Recombinant Protein
 • Vimentin Antibody
 • suPAR (soluble urokinase-type plasminogen activator receptor... ELISA Kit
 • Stem Cell Factor (rSCF) Recombinant Protein
 • 4 Hydroxynonenal (4 HNE) ELISA Kit
 • Paxillin (PXN) Antibody
 • REG1beta ELISA Kit
 • OGN cDNA Clone
 • ACTH (Adrenocorticotrophic Hormone) Antibody
 • N-acetyl-D-glucosamine kinase (NAGK) Antibody
 • CD248 Blocking Peptide
 downarrow more ...
separator

S100A12 elisa kit product blog

separator
Posted on 2017-03-04 02:23:20 by mybiosource_staff
Share it on ...     
separator
separator
Tags: ELISA Kit; Human ELISA Kit; S100A12 elisa kit; S100A12; S100 Calcium Binding Protein A12 (S100A12);
separator
The Human S100A12 s100a12 (Catalog #MBS455912) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS455912 ELISA Kit recognizes Human (General) S100A12.

The S100A12 s100a12 product has the following accession number(s) (GI #5032059) (NCBI Accession #NP_005612.1) (Uniprot Accession #P80511). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


(Or you can also download the PDF Manual for complete product instructions).

Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Human S100 Calcium Binding Protein A12 (S100A12) ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only) S100A12.

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of S100A12 in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to S100A12. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to S100A12. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain S100A12, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of S100A12 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Assay Type: Sandwich
Detection Range: 78.125-5000pg/mL
Sensitivity: 34pg/mL. Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level S100A12 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level S100A12 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%. Human S100 Calcium Binding Protein A12 (S100A12) ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. Atherosclerosis, Cardiovascular Diseases, Colitis, Diabetes Mellitus, Immune System Diseases, Inflammation, Kidney Diseases, Necrosis, Nervous System Diseases, Vascular Diseases are some of the diseases may be linked to Human S100 Calcium Binding Protein A12 (S100A12) ELISA Kit. The following patways have been known to be associated with this gene. Adipose Tissue, Blood, Bone Marrow, Heart, Intestine, Kidney, Liver, Spleen tissues are correlated with this protein. S100A12 also interacts with the following gene(s): AGER, CACYBP, HMGB1, NOX1, S100A7.
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions