Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource  rightarrow Assay Kit Blog  rightarrow Rt3 elisa kit  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Blogs moreseparator
separator
 • GAP43 elisa kit blog
 • ETRA elisa kit blog
 • ANXA6 elisa kit blog
 • OVA slgE elisa kit blog
 • CCDC144B elisa kit blog
 • PAG-1 elisa kit blog
 • TIMP-3 elisa kit blog
 • CRT elisa kit blog
 • FBN2 elisa kit blog
 • CNTNAP3 elisa kit blog
 • GDPD1 elisa kit blog
 • celf2 elisa kit blog
 • FASL elisa kit blog
 • COMT elisa kit blog
 • OC/BGP elisa kit blog
 • CTNNb1 clia kit blog
 • HCRTR2 elisa kit blog
 • GLUT2 elisa kit blog
 • TLR3 elisa kit blog
 • SDF1 elisa kit blog
separator
Blog Categories separator
separator
 • Assay Kit Blogs
 • Biochemical Blogs
 • cDNA Clone Blogs
 • Monoclonal Antibody Blogs
 • Polyclonal Antibody Blogs
 • Rec/Purified Protein Blogs
 • Popular Product Blogs
separator
Our Best Sellers moreseparator
separator
 • IRAK4 Antibody
 • Trans-activator protein BZLF1 Recombinant Protein
 • 3 beta-hydroxysteroid dehydrogenase-Delta 5-->4-isomerase (H... Recombinant Protein
 • alpha 1-acid glycoprotein (OGCHI) ELISA Kit
 • ALDH3B1 Antibody
 • MARVEL domain containing 2 (MARVELD2) Antibody
 • phosphofurin acidic cluster sorting protein 2 (pacs2) Antibody
 • PSP Antibody
 • Dexamethasone (DXM) ELISA Kit
 • Glycoprotein Antibody (GP) ELISA Kit
 • L-lactate dehydrogenase B chain (LDHB) Recombinant Protein
 • CT ELISA Kit
 • Activating Transcription Factor 3 (ATF3) ELISA Kit
 • 4,5-DOPA dioxygenase extradiol (DODA) Recombinant Protein
 downarrow more ...
separator

Rt3 elisa kit product blog

separator
Posted on 2017-12-14 08:31:35 by mybiosource_staff
Share it on ...     
separator
separator
Tags: ELISA Kit; Rat ELISA Kit; RT3 elisa kit; RT3; Reverse Triiodothyronine;
separator
The Rat Rt3 n/a (Catalog #MBS765603) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS765603 ELISA Kit recognizes Rat (Rattus, General) Rt3.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


(Or you can also download the PDF Manual for complete product instructions).

Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Rat Reverse Triiodothyronine ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only) Rt3.

Principle of the Assay: This kit was based on Competitive-ELISA detection method. The microtiter plate provided in this kit has been pre-coated with Rt3. During the reaction, Rt3 in the sample or standard competes with a fixed amount of Rt3 on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to Rt3. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Rt3 in the samples is then determined by comparing the O D of the samples to the standard curve.

Samples: Serum, plasma, tissue homogenates and other biological fluids
Assay Type: Competitive
Detection Range: 93.75-6000pg/ml
Sensitivity: <56.25pg/ml. Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Rt3 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Rt3 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%. Rat Reverse Triiodothyronine ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions