|The Monkey RPA-1 n/a (Catalog #MBS750843) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS750843 ELISA Kit recognizes Monkey (Rhesus Macaque, Rhesus Monkey, General) RPA-1.
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Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Monkey Renal Papillary Antigen-1 ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Principle of the assay: RPA-1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-RPA-1 antibody and an RPA-1-HRP conjugate. The assay sample and buffer are incubated together with RPA-1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the RPA-1 concentration since RPA-1 from samples and RPA-1-HRP conjugate compete for the anti-RPA-1 antibody binding site. Since the number of sites is limited, as more sites are occupied by RPA-1 from the sample, fewer sites are left to bind RPA-1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The RPA-1 concentration in each sample is interpolated from this standard curve.
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type: Competitive
Sensitivity: 1.0 pg/ml. Intended Uses: This RPA-1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey RPA-1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Monkey Renal Papillary Antigen-1 ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.