RBP elisa kit product blog
Tags: ELISA Kit; Human ELISA Kit; RBP; RBP elisa kit; Retinol Binding Protein/ RBP4;
The Human RBP rbp4 (Catalog #MBS495091) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS495091 ELISA Kit recognizes Human RBP.The RBP rbp4 product has the following accession number(s) (GI #119570453) (NCBI Accession #EAW50068.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Intended Uses: The Murine TNF-alpha ELISA is to be used for the in-vitro quantitative determination of murine tumor necrosis factor-alpha (mTNFalpha) in murine serum, buffered solutions or cell culture medium. The assay will recognize both natural and recombinant murine TNF-alpha.
Principle of the Assay: The murine TNF-alpha Kit is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (ELISA). A monoclonal antibody specific for mTNFalpha has been coated onto the wells of the microtiter strips provided. The antigen and a biotinylated polyclonal antibody specific for mTNFalpha are simultaneously incubated. Revelation step includes Streptavidin-Horse Radish peroxidase and TMB as chromogen.
Samples: Murine Serum, Buffered Solutions Or Cell Culture Medium
Assay Type: Quantitative Sandwich
Sensitivity: < 10.7 pg/ml. Intra-assay Precision: Reproducibility within the assay was evaluated in three independent experiments. Each assay was carried out with 6 replicates (3 duplicates) of 6 different samples containing different concentrations of TNFalpha. Data below show the mean concentration and the coefficient of variation for each sample. The overall intraassay coefficient of variation has been calculated to be 5%
Inter-assay Precision: Assay to assay reproducibility within one laboratory will be evaluated in three independent experiments. Each assay will be carried out with 6 replicates (3 duplicates) in 2 murine pooled serum, 2 in RPMI and 2 in standard diluent with samples containing different concentrations of TNFalpha. 2 standard curves were run on each plate. The calculated overall coefficient of variation was 9.4%. Retinol Binding Protein (RBP) / RBP4 ELISA or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. RBP also interacts with the following gene(s): AHSG, ALB, AMBP, CYP2C9, PSMB1, RETN, RPE65, STRA6, TTR. Fatty Liver, Glucose Intolerance, Hyperglycemia, Inflammation, Kidney Diseases, Liver Diseases, Necrosis, Polycystic Ovary Syndrome, Proteinuria, Weight Loss are some of the diseases may be linked to Retinol Binding Protein (RBP) / RBP4 ELISA. The following patways have been known to be associated with this gene. Bone, Brain, Connective Tissue, Embryonic Tissue, Eye, Heart, Kidney, Liver, Muscle, Pancreas tissues are correlated with this protein.