Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource  rightarrow Assay Kit Blog  rightarrow RANKL elisa kit  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Blogs moreseparator
separator
 • MYD88 elisa kit blog
 • GYPA elisa kit blog
 • FSTL3 elisa kit blog
 • alpha1-AT elisa kit blog
 • CIDEC / FSP27 elisa kit blog
 • CENPF elisa kit blog
 • ZNF594 elisa kit blog
 • PLG elisa kit blog
 • ANUA-IgG elisa kit blog
 • PDGFC elisa kit blog
 • alphaMACR elisa kit blog
 • MAPK14 elisa kit blog
 • CGRP elisa kit blog
 • 8-iso-PGF2a elisa kit blog
 • TNP1 elisa kit blog
 • E2/UBCE elisa kit blog
 • 5-LO elisa kit blog
 • ACVR1 elisa kit blog
 • ATP5J elisa kit blog
 • GDF7 elisa kit blog
separator
Blog Categories separator
separator
 • Assay Kit Blogs
 • Biochemical Blogs
 • cDNA Clone Blogs
 • Monoclonal Antibody Blogs
 • Polyclonal Antibody Blogs
 • Rec/Purified Protein Blogs
 • Popular Product Blogs
separator
Our Best Sellers moreseparator
separator
 • sema domain, immunoglobulin domain (Ig) , short basic domain... ELISA Kit
 • SSH2 Antibody
 • FAS Antibody
 • Rantes (CCL5) Recombinant Protein
 • CLIC4 Antibody
 • P-Cadherin (CDH3) ELISA Kit
 • HRP (Horseradish Peroxidase) Antibody
 • YWHAG Antibody
 • Kallikrein (KLKB1) Protein
 • Vesicle-associated membrane protein 2 (VAMP2) Recombinant Protein
 • IgE Antibody
 • Ecto-NOX disulfide-thiol exchanger 1 (ENOX1) Recombinant Protein
 • Rabies virus Matrix protein Antibody
 • Colicin-E1 (cea) Recombinant Protein
 downarrow more ...
separator

RANKL elisa kit product blog

separator
Posted on 2015-07-20 11:46:56 by mybiosource_staff
Share it on ...     
separator
separator
Tags: ELISA Kit; Goat ELISA Kit; Receptor Activator of Nuclear Factor Kb Ligand; RANKL elisa kit; RANKL;
separator
The Goat RANKL tnfsf11 (Catalog #MBS743584) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS743584 ELISA Kit recognizes Goat (Caprine, General) RANKL.

The RANKL tnfsf11 product has the following accession number(s) (GI #2612922) (NCBI Accession #AAB86811.1) (Uniprot Accession #O14788). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


(Or you can also download the PDF Manual for complete product instructions).

Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Goat Receptor Activator of Nuclear Factor Kb Ligand ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only) RANKL.

Principle of the Assay: RANKL ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-RANKL antibody and an RANKL-HRP conjugate. The assay sample and buffer are incubated together with RANKL-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the RANKL concentration since RANKL from samples and RANKL-HRP conjugate compete for the anti-RANKL antibody binding site. Since the number of sites is limited, as more sites are occupied by RANKL from the sample, fewer sites are left to bind RANKL-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The RANKL concentration in each sample is interpolated from this standard curve.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type: Competitive
Sensitivity: 0.1 ng/mL. Intended Uses: This RANKL ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Goat RANKL. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Goat Receptor Activator of Nuclear Factor Kb Ligand ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. RANKL also interacts with the following gene(s): AKT1, MAPK1, MAPK3, MAPK8, MMP1, MMP3, NFKB1, TNFRSF11A, TNFRSF11B, TRAF6.
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions