QuickTiter Lentivirus quantitation kit product blog
Tags: Quantitation Kit; QuickTiter Lentivirus; QuickTiter Lentivirus quantitation kit;
The QuickTiter Lentivirus n/a (Catalog #MBS168453) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase.To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given assay kit. We\'ve tested the QuickTiter Lentivirus Quantitation Kit with the following immunoassay(s):
Testing Data
Testing Data #2
Background: Lentivirus vector based on the human immunodeficiency virus-1 (HIV-1) has become a promising vector for gene transfer studies. The advantageous feature of lentivirus vector is the ability of gene transfer and integration into dividing and non-dividing cells. The pseudotyped envelope with vesicular stomatitis virus envelope G (VSV-G) protein broadens the target cell range. Lentiviral vectors have been shown to deliver genes to neurons, lymphocytes and macrophages, cell types that previous retrovirus vectors could not be used. Lentiviral vectors have also proven to be effective in transducing brain, liver, muscle, and retina in vivo without toxicity or immune responses. Recently, the lentivirus system is widely used to integrate siRNA efficiently in a wide variety of cell lines and primary cells both in vitro and in vivo. Lentivirus particles are produced from 293T cells through transient transfection of 3 or 4 plasmids that encodes for the components of the virion. Viral medium containing viral particles produced by packaging cells within 48-72 hr can be harvested. To ensure that pseudoviral medium is viable, and to control the number of copies of integrated viral constructs per target cell, the viral titer needs to be determined before proceeding with transduction experiments. Viral titer can be determined by transduction of HT-1080 or Hela cells, and followed by antibiotic selection of stable clones. However, it takes weeks to generate sizable stable cell colonies for counting and calculating the titer results. proprietary QuickTiter Lentiviral Quantitation Kit does not involve cell infection; instead it specifically measures the viral nucleic acid content of purified viruses or unpurified viral supernatant sample (See Test Principle). In the case of unpurified viral supernatant, the kit is especially useful for determining the supernatant titer before the transduction step. The kit has detection sensitivity limit of 1 X 1010 VP/mL, which is sufficient for mid or high-titer lentivirus sample. The entire procedure takes about 45 to 60 minutes. Each kit provides sufficient reagents to perform up 20 tests for viral samples and controls. QuickTiter Lentiviral Quantitation Kit provides an efficient system for rapid quantitation of lentivirus titer for both viral supernatant and purified virus. The system may be adapted to quantitation of other viral types, such as retrovirus and adenovirus.