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PTX3 elisa kit product blog

Posted on 2015-08-01 16:37:05 by mybiosource_staff
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Tags: ELISA Kit; Human ELISA Kit; PTX3 elisa kit; PTX3; PTX3/Pentraxin 3;
The Human PTX3 ptx3 (Catalog #MBS176542) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS176542 ELISA Kit recognizes Human (General) PTX3.

The PTX3 ptx3 product has the following accession number(s) (GI #296452984) (NCBI Accession #P26022.3) (Uniprot Accession #P26022). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

(Or you can also download the PDF Manual for complete product instructions).

Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Human PTX3/Pentraxin 3 PicoKine ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
 Typical Testing Data/Standard Curve (for reference only) PTX3.

Principle of the Assay: Human PTX3/Pentraxin 3 ELISA Kit was based on standard sandwich enzyme-linked immunesorbent assay technology. A monoclonal antibody from mouse specific for PTX3/Pentraxin 3 has been precoated onto 96-well plates. Standards(Expression system for standard: NSO; Immunogen sequence: E18-S381) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for PTX3/Pentraxin 3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human PTX3/Pentraxin 3 amount of sample captured in plate.

Background: PTX3(Pentraxin 3) is a member of the pentraxin superfamily. This super family characterized by cyclic multimeric structure. The PTX3 gene is mapped to 3q25.32. The predicted 381-amino acid PTX3 protein has homology to the pentraxin protein family. Significant levels of PTX3 were detected in plasma of neutropenic patients with systemic A. PTX3 is effective in preventing CMV infection and reactivation, as well as subsequent Aspergillus infection. PTX3 activates the classical pathway of complementactivation and facilitates pathogen recognition by macrophages and DCs.

Assay Type: Quantitative Sandwich
Detection Range: 312pg/ml-20000pg/ml
Sensitivity: <10pg/ml. Human PTX3/Pentraxin 3 PicoKine ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. PTX3 also interacts with the following gene(s): C1S, FGF2, IL1B. Atherosclerosis, Heart Diseases, Heart Failure, Inflammation, Kidney Diseases, Lung Diseases, Myocardial Infarction, Necrosis, Nervous System Diseases, Skin Diseases are some of the diseases may be linked to Human PTX3/Pentraxin 3 PicoKine ELISA Kit. Bone, Brain, Connective Tissue, Embryonic Tissue, Heart, Kidney, Liver, Lung, Muscle, Vascular tissues are correlated with this protein.
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