PSA elisa kit product blog
Tags: ELISA Kit; Human ELISA Kit; PSA elisa kit; PSA;
The Human PSA n/a (Catalog #MBS494876) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS494876 ELISA Kit recognizes Human PSA.To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Intended Uses: The MyBioSource Estrone-3-Sulfate equine is a competitive enzyme immunoassay for the measurement of estrone-3-sulfate in mare serum. For Research Use Only,.
Principle of the Assay: The MyBioSource Estrone-3-Sulfate equine ELISA test kit is a solid phase enzyme immunoassay (ELISA) in the microplate format, designed for the measurement of estrone-3-sulfate. The microplate is coated with a polyclonal antibody specific for estrone-3-sulfate. Calibrators and samples as well as a biotin-labeled estrone-3-sulfate are pipetted into the antibody coated microplate. During one hour incubation the estrone-3-sulfate of the calibrator/sample competes with the biotin-labeled estrone-sulfate for the binding sites of the antibody fixed on the inner surface of the wells. Afterwards, horseradish peroxidase-labeled streptavidine is added. During an 30 minutes incubation, this enzyme-labeled streptavidine binds to the biotinylated estrone-3-sulfate. All wells are washed to remove excess of non-bound enzyme-labeled streptavidine. A chromogenic substrate, TMB (3,3\',5,5\'-Tetra-Methyl-Benzidine), is added to all wells. During a 30 minutes incubation, the substrate is converted to a colored end product (blue) by the fixed enzyme. Enzyme reaction is stopped by dispensing of hydrochloric acid as stop solution (change from blue to yellow). The color intensity is inversely related to the concentration of estrone-3-sulfate present in the sample. The optical density of the color solution is measured with a microplate reader at 450 nm. Bi-chromatic measurement with a 600-690 nm reference filter is recommended.
Samples: Mare Serum
Assay Type: Competitive
Sensitivity: 0.14 ng/ml. Free PSA ELISA, 96 well or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.