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PRO-NGF1 elisa kit product blog

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Posted on 2016-02-19 19:16:49 by mybiosource_staff
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Tags: ELISA Kit; Mouse ELISA Kit; pro-NGF1 elisa kit; pro-NGF1; Pro-Nerve Growth Factor 1;
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The Mouse PRO-NGF1 n/a (Catalog #MBS046227) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS046227 ELISA Kit recognizes Mouse (Murine, General) PRO-NGF1.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


(Or you can also download the PDF Manual for complete product instructions).

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated PRO-NGF1 concentrations in Mouse serum, plasma and other body fluids. Using Purified Mouse PRO-NGF1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add PRO-NGF1 and PRO-NGF1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of PRO-NGF1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

PRO-NGF1 n/a

Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids
Assay Type: Sandwich
Detection Range: 31.2pg/ml-1000pg/ml.
Sensitivity: 5.0pg/ml. Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated PRO-NGF1 concentrations in Mouse serum, plasma and other body fluids. Using Purified Mouse PRO-NGF1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add PRO-NGF1 and PRO-NGF1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of PRO-NGF1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Intra-assay Precision: Intra-assay CV (%) is less than 15%.
Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean 100]. Mouse Pro-Nerve Growth Factor 1 ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.
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