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Pro-MMP-1 clia kit product blog

Posted on 2015-11-17 17:55:19 by mybiosource_staff
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Tags: Pro-MMP-1; CLIA Kit; Human CLIA Kit; Pro-MMP-1 (pro-Matrix Metalloproteinase 1); Pro-MMP-1 clia kit;
The Human Pro-MMP-1 n/a (Catalog #MBS2532079) is a CLIA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS2532079 CLIA Kit recognizes Human (General) Pro-MMP-1.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

(Or you can also download the PDF Manual for complete product instructions).

Please refer to the product datasheet for known applications of a given clia kit. We\'ve tested the Human Pro-MMP-1 (pro-Matrix Metalloproteinase 1) CLIA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only) Pro-MMP-1.

Intended Uses: This CLIA kit applies to the in vitro quantitative determination of Pro-MMP-1 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Pro-MMP-1. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Pro-MMP-1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Pro-MMP-1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Pro-MMP-1. You can calculate the concentration of Pro-MMP-1 in the samples by comparing the RLU value of the samples to the standard curve.

Samples: Serum, plasma and other biological fluids
Assay Type: Quantitative Sandwich
Detection Range: 31.25-2000ng/mL
Sensitivity: 18.75ng/mL.
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