PRL elisa kit product blog
Tags: ELISA Kit; Prolactin; PRL elisa kit; PRL;
The General PRL prl (Catalog #MBS135500) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS135500 ELISA Kit recognizes General PRL.The PRL prl product has the following accession number(s) (GI #130934) (NCBI Accession #P06879.1) (Uniprot Accession #P06879). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Mouse Prolactin ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Intended Uses: This mouse prolactin antigen assay is intended for the quantitative determination of prolactin antigen in mouse plasma.
Principle of the Assay: Mouse prolactin will bind to the affinity purified capture antibody coated on the microtiter plate. After appropriate washing steps, biotinlyated anti-mouse prolactin primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody, which is proportional to the total prolactin present in the samples, is reacted with peroxidase-conjugated streptavidin. Following an additional washing step, TMB substrate is used for color development which is measured at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of mouse prolactin. Color development is proportional to the concentration of prolactin in the samples.
Background: Mouse prolactin (PRL) is a 197 aa, 23kD peptide hormone [1] that is secreted primarily by the pituitary gland in both males and females, though its major roles are in pregnancy and lactation [2,3]. Prolactin may have a role in breast cancer development, with higher prolactin levels correlating with postmenopausal breast cancer risk [4].
Samples: Mouse plasma. Mouse Prolactin ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. Brain, Embryonic Tissue, Pituitary Gland, Spinal Cord, Uncharacterized Tissue tissues are correlated with this protein. The following patways have been known to be associated with this gene.