PR3-ANCA elisa kit product blog
Tags: ELISA Kit; Rabbit ELISA Kit; Proteinase 3 antineutrophil cytoplasmic antibody; PR3-ANCA elisa kit; PR3-ANCA;
The Rabbit PR3-ANCA n/a (Catalog #MBS720178) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS720178 ELISA Kit recognizes Rabbit PR3-ANCA.To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Rabbit Proteinase 3 antineutrophil cytoplasmic antibody ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Principle of the assay: PR3-ANCA ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PR3-ANCA antibody and an PR3-ANCA-HRP conjugate. The assay sample and buffer are incubated together with PR3-ANCA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PR3-ANCA concentration since PR3-ANCA from samples and PR3-ANCA-HRP conjugate compete for the anti-PR3-ANCA antibody binding site. Since the number of sites is limited, as more sites are occupied by PR3-ANCA from the sample, fewer sites are left to bind PR3-ANCA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PR3-ANCA concentration in each sample is interpolated from this standard curve.
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type: Competitive
Sensitivity: 0.1 ng/mL. Intended Uses: This PR3-ANCA ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rabbit PR3-ANCA. This ELISA kit for research use only, not for therapeutic applications!. Rabbit Proteinase 3 antineutrophil cytoplasmic antibody ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.