PNP assay kit product blog
Tags: PNP; Assay Kit; Purine Nucleoside Phosphorylase; PNP assay kit;
The PNP pnp (Catalog #MBS841447) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase.Detection of Purine Nucleoside Phosphorylase activity in variety of samples. Researchers should empirically determine the suitability of the PNP pnp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The PNP pnp product has the following accession number(s) (GI #157168362) (NCBI Accession #NP_000261.2). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given assay kit. We\'ve tested the Purine Nucleoside Phosphorylase Activity Assay Kit (Fluorometric) with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Product Information: Purine Nucleoside Phosphorylase Activity Assay Kit (Fluorometric): Rapid & convenient assay to measure PNP activity in tissues, cells, and of recombinant protein. Limit of quantification is 0.005 uU recombinant Purine Nucleoside Phosphorylase. 100 assays
Background: Purine Nucleoside Phosphorylase (PNP) (E.C. 2.4.2.1.) is an enzyme involved in purine metabolism and it catalyzes the cleavage of the glycosidic bond of ribo- or deoxyribonucleosides, in the presence of inorganic phosphate as a second substrate, to generate the purine base and ribose-1-phosphate or deoxyribose-1-phosphate. It is one of the enzymes of the nucleotide salvage pathways that allows the cell to produce nucleotide monophosphates when the de novo synthesis pathway has been interrupted or is non-existent (as is the case in the brain). PNP is a cytosolic enzyme. PNP deficiency disease leads to toxic buildup of deoxyguanosine in T-cells leading to T-lymphocytopenia with no apparent effects on B-lymphocyte function. Inhibition of PNP is an important target for chemotherapeutic applications and treatment of T- cell mediated autoimmune diseases. PNP deficiency is also associated with neurological problems. In MyBioSource\'s Purine Nucleoside Phosphorylase Activity Assay, hypoxanthine formed from the breakdown of inosine is detected via a multi-step reaction, resulting in the generation of an intermediate that reacts with the PNP Probe. The fluorescent product is measured at Ex/Em = 535/587 nm. Limit of quantification is 0.005 uU recombinant Purine Nucleoside Phosphorylase.
Summary: Detection: Fluorescence (Ex/Em = 535/587 nm)
Application: Detection of Purine Nucleoside Phosphorylase activity in variety of samples.
Samples: Purified recombinant protein
Cell and tissue lysate. Detection Method: Fluorescence (Ex/Em = 535/587 nm)
Features & Benefits: Rapid, simple & convenient
Limit of quantification is 0.005 uU recombinant Purine Nucleoside Phosphorylase. Blood, Bone, Brain, Heart, Kidney, Liver, Lung, Skin, Spleen, Vascular tissues are correlated with this protein. Brain Diseases, Cardiovascular Diseases, Fibrosis, Heart Diseases, Hyperplasia, Inflammation, Liver Neoplasms, Necrosis, Neurologic Manifestations, Purine Nucleoside Phosphorylase Deficiency are some of the diseases may be linked to Purine Nucleoside Phosphorylase Activity Assay Kit (Fluorometric).