|The Human PNOC pnoc (Catalog #MBS763663) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS763663 ELISA Kit recognizes Human (General) PNOC.
The PNOC pnoc product has the following accession number(s) (GI #6981378) (NCBI Accession #NP_037139.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
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Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Human Prepronociceptin ELISA Kit with the following immunoassay(s):
Typical Testing Data
Typical Standard Curve
Principle of the Assay: This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with an antibody specific to PNOC. During the reaction, PNOC in the sample or standard competes with a fixed amount of biotin-labeled PNOC for sites on a pre-coated Monoclonal antibody specific to PNOC. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of PNOC in the samples is then determined by comparing the OD. of the samples to the standard curve.
Samples: Serum, plasma and other biological fluids.
Assay Type: Competitive ELISA, Coated with Antigen
Detection Range: 0.156-10ng/ml
Sensitivity: 0.094ng/ml. Human Prepronociceptin ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. Brain Diseases, Breast Neoplasms, Disease Models, Animal, Hyperalgesia, Inflammation, Memory Disorders, Necrosis, Nervous System Diseases, Pain, Seizures are some of the diseases may be linked to Human Prepronociceptin ELISA Kit. Brain tissues are correlated with this protein. The following patways have been known to be associated with this gene. PNOC also interacts with the following gene(s): Npy, Oprd1, Oprk1, Oprl1, Oprm1, Pdyn, Penk, Pomc.