PNLIP assay kit product blog
Tags: Lipase; PNLIP; Assay Kit; PNLIP assay kit;
The PNLIP pnlip (Catalog #MBS841708) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase.The PNLIP pnlip product has the following accession number(s) (GI #190140) (NCBI Accession #AAA60129.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given assay kit. We\'ve tested the Lipase Activity Colorimetric Assay Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Product Information: Lipase Activity Assay Kit: High-Throughput Suitable Colorimetric Assay for Measuring Lipase Activity in various biological Samples such as Serum, Tissues, Cells etc. Simple, Sensitive & Accurate. Detection Limit: 0.02 mU. 100 Assays.
Background: Lipases perform essential roles in the digestion, transport and processing of dietary lipids (e.g. fats and oils) in living organisms. In humans, pancreatic lipase is the key enzyme responsible for breaking down fats in the digestive system by converting triglyceride to monoglyceride and free fatty acid. Pancreatic lipase monitoring is also used to help identify Crohn\'s disease, cystic fibrosis and celiac disease. Damage to the pancreas can exhibit a 5-10 fold increase of serum lipase levels within 24 to 48 hours. In MyBioSource\'s Lipase Assay Kit, lipase hydrolyzes a triglyceride substrate to form glycerol which is quantified enzymatically by via monitoring a linked change in the OxiRed probe absorbance (lambda=570nm). This assay is rapid, simple, sensitive, and reliable, as well as, suitable for high throughput activity screening of lipase. This kit detects lipase activity as low as 10mU per well.
Summary: Detection method-Absorbance (570 nm)
Species reactivity- Mammalian
Applications- This kit detects lipase activity as low as 10mU per well.
Samples: Cell and tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids, growth medium, food, etc. Features & Benefits: Simple procedure; takes ~ 60-90 minutes
Fast and convenient
Sensitive assays for measuring Lipase activity in various biological samples. Cardiovascular Diseases, Diabetes Mellitus, Fibrosis, Heart Diseases, Hyperlipidemias, Hypertriglyceridemia, Insulin Resistance, Necrosis, Pancreatitis, Weight Loss are some of the diseases may be linked to Lipase Activity Colorimetric Assay Kit. The following patways have been known to be associated with this gene. Adipose Tissue, Connective Tissue, Heart, Liver, Pancreas tissues are correlated with this protein. PNLIP also interacts with the following gene(s): CEL, CLPS.