|The PLTP Inhibitor Drug Screening Kit n/a (Catalog #MBS841737) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase.
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Product Information: Plasma Phospholipid Transfer Protein (PLTP) Inhibitor Drug Screening Kit: Fluorometric Assay for Screening PLTP Inhibitors within 30-60 min. Simple, Convenient & Reliable. 100 Assays.
Background: Plasma phospholipid transfer protein (PLTP) is thought to play a major role in the facilitated transfer of phospholipids between lipoproteins and in the modulation of high-density lipoprotein (HDL) particle size and composition. PLTP-facilitated lipid transfer activity is related to HDL and LDL metabolism, as well as lipoprotein lipase activity, adiposity, and insulin resistance. The PLTP Drug Screening Kit utilizes rabbit serum as PLTP activity source to screen PLTP inhibitors. The assay uses a donor molecule containing a fluorescent self-quenched phospholipid that is transferred to an acceptor molecule in the presence of PLTP. PLTP-mediated transfer of the fluorescent phospholipid to the acceptor molecule results in an increase in fluorescence (Ex/ Em- 465/ 535 nm). Inhibitor of PLTP will inhibit the lipid transfer and therefore decrease fluorescence intensity. The kit provides sufficient reagents for 100 PLTP inhibitor screening assays.
Summary: Detection method- Fluorescence (Ex 465/535 nm)
Species reactivity- Mammalian.
Samples: Cell and tissue culture supernatants, urine, plasma, serum, as well as many other biological fluids. Features & Benefits: Simple procedure; takes ~40 minutes
Fast and convenient
Provided all necessary buffers and reagents, as well as positive control for accurate assay of activity of plasma phospholipid transfer protein (PLTP).