PLGF elisa kit product blog
Tags: ELISA Kit; Human ELISA Kit; PLGF; PLGF elisa kit;
The Human PLGF pgf (Catalog #MBS494813) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS494813 ELISA Kit recognizes Human PLGF.The PLGF pgf product has the following accession number(s) (GI #652698534) (NCBI Accession #NP_001280572.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the PLGF ELISA with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Intended Uses: For detection of PDGF-AB in rat serum, plasma, body fluids, tissue lysates or cell culture supernatants. For research use only.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-PDGF-AB polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-PDGF-AB polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the PDGF-AB amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of PDGF-AB can be calculated
Background/Introduction: Platelet-derived growth factor (PDGF) was one of the first growth factors characterized. and also a potent mitogen for cells of mesenchymal origin, including smooth muscle cells and glial cells. It regulates cell growth and division, In particular, it plays a significant role in blood vessel formation (angiogenesis). It is produced by a plethora of cells, and synthesized, stored, released by platelets upon activation. In chemical terms, PSGF is dimeric glycoprotein composed of two A (-AA) or two B (-BB) chains or a combination of the two (-AB). It is found that initial PDGF-AB interaction with the alpha PDGFR induces conformational changes in the ligand or receptor that facilitates efficient recruitment of beta PDGFR by this PDGF isoform.
Samples: Rat Serum, Plasma, Body Fluids, Tissue Lysates Or Cell Culture Supernates
Assay Type: Sandwich
Detection Range: 31.2pg/ml-2000pg/ml
Sensitivity: <3pg/ml. PLGF ELISA or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. Brain Diseases, Cardiovascular Diseases, Death, Disease Models, Animal, Heart Diseases, Hypertension, Inflammation, Kidney Diseases, Lung Diseases, Necrosis are some of the diseases may be linked to PLGF ELISA. Brain, Embryonic Tissue, Heart, Kidney, Liver, Lung, Muscle, Ovary, Placenta, Uterus tissues are correlated with this protein. PLGF also interacts with the following gene(s): AKT1, EDN1, FLT1, FLT4, KDR, NRP1, PIK3CA, VEGFA. The following patways have been known to be associated with this gene.