Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource  rightarrow Assay Kit Blog  rightarrow PLGF elisa kit  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Blogs moreseparator
separator
 • GALR2 elisa kit blog
 • SFT elisa kit blog
 • MAPK8 elisa kit blog
 • ZBTB8A elisa kit blog
 • RLP-C elisa kit blog
 • INTS6 elisa kit blog
 • SCG2 elisa kit blog
 • ADP elisa kit blog
 • FGL2 elisa kit blog
 • mmu-mir-200c-3p pcr kit blog
 • ADV-IgM elisa kit blog
 • ZNF294 elisa kit blog
 • CHRNA7 elisa kit blog
 • CA elisa kit blog
 • PLIN1 elisa kit blog
 • CLEC3B elisa kit blog
 • M-ACHR elisa kit blog
 • ALCAM elisa kit blog
 • SCNN1A elisa kit blog
 • CXCL2 elisa kit blog
separator
Blog Categories separator
separator
 • Assay Kit Blogs
 • Biochemical Blogs
 • cDNA Clone Blogs
 • Monoclonal Antibody Blogs
 • Polyclonal Antibody Blogs
 • Rec/Purified Protein Blogs
 • Popular Product Blogs
separator
Our Best Sellers moreseparator
separator
 • Iodotyrosine dehalogenase 1 (IYD) ELISA Kit
 • Ribonuclease A7 (RNASE7) ELISA Kit
 • MINPP1 Antibody
 • Endothelin 1 (EDN1) ELISA Kit
 • Embryonic Stem MESPU30 (M30) ELISA Kit
 • Matrix Metalloproteinase 11 (MMP-11) ELISA Kit
 • SHMT2 Antibody
 • Ankyrin repeat and SOCS box protein 12 (Asb12) Recombinant Protein
 • JTB cDNA Clone
 • Galactoside 3 (4)-L-fucosyltransferase (FUT3) Recombinant Protein
 • Tobacco Etch Virus Protease, mutant, GST-tag Recombinant Protein
 • Notch Homolog 3 (NOTCH3) Recombinant Protein
 • Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mi... Recombinant Protein
 • MFGE8-Lactadherin ELISA Kit
 downarrow more ...
separator

PLGF elisa kit product blog

separator
Posted on 2015-12-17 15:23:14 by mybiosource_staff
Share it on ...     
separator
separator
Tags: ELISA Kit; Mouse ELISA Kit; PLGF; Placenta Growth Factor (PLGF); PLGF elisa kit;
separator
The Mouse PLGF pgf (Catalog #MBS2021431) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS2021431 ELISA Kit recognizes Mouse (Murine, General) PLGF.

The PLGF pgf product has the following accession number(s) (GI #6679289) (NCBI Accession #NP_032853.1) (Uniprot Accession #P49764). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


(Or you can also download the PDF Manual for complete product instructions).

Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Placenta Growth Factor (PLGF) ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only) PLGF.

Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Placenta Growth Factor (PLGF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Placenta Growth Factor (PLGF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Placenta Growth Factor (PLGF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Placenta Growth Factor (PLGF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Samples: Serum, plasma, tissue homogenates and other biological fluids
Assay Type: Sandwich
Detection Range: 7.81-500pg/mL
Sensitivity: Typically less than 2.7pg/mL. Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Placenta Growth Factor (PLGF) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Placenta Growth Factor (PLGF) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Assay Procedure Summary: 1. Prepare all reagents, samples and standards;
2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;
3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;
4. Aspirate and wash 3 times;
5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;
6. Aspirate and wash 5 times;
7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;
8. Add 50uL Stop Solution. Read at 450nm immediately. Placenta Growth Factor (PLGF) ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. Brain Diseases, Cardiovascular Diseases, Death, Disease Models, Animal, Heart Diseases, Hypertension, Inflammation, Kidney Diseases, Lung Diseases, Necrosis are some of the diseases may be linked to Placenta Growth Factor (PLGF) ELISA Kit. Brain, Embryonic Tissue, Extraembryonic Tissue, Eye, Intestine, Liver, Lung, Pituitary Gland, Spinal Cord, Stomach tissues are correlated with this protein. The following patways have been known to be associated with this gene. PLGF also interacts with the following gene(s): Akt1, Figf, Flt1, Flt4, Kdr, Nrp1, Vegfa, Vegfb, Vegfc.
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions