PLAU elisa kit product blog
Tags: ELISA Kit; PLAU; PLAU elisa kit; uPA functional assay;
The General PLAU plau (Catalog #MBS135544) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS135544 ELISA Kit recognizes General PLAU.The PLAU plau product has the following accession number(s) (GI #267251) (NCBI Accession #P29598.1) (Uniprot Accession #P29598). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Active rat uPA functional assay ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Intended Uses: Rat uPA activity assay is intended for the quantitative determination of active urokinase plasminogen activator in rat plasma. For research use only.
Principle of the Assay: Functionally active uPA will bind to the biotinylated human PAI-1 coated on the microtiter plate. Only free active enzyme will react with the PAI-1 on the plate. Inactive or complexed enzyme will not be detected. After appropriate washing steps, anti-rat uPA primary antibody binds to the captured enzyme. Excess antibody is washed away and bound monoclonal antibody is then reacted with the secondary antibody conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of uPA.
Background: Urokinase plasminogen activator is a serine protease that activates plasminogen to plasmin in the blood fibrinolytic system. It is also implicated in events related to cell invasion/migration [3].
Samples: Rat plasma. Intra-assay Precision: These studies are currently in progress. Please contact us for more information.
Inter-assay Precision: These studies are currently in progress. Please contact us for more information. Active rat uPA functional assay ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. Adipose Tissue, Connective Tissue, Kidney, Lung, Muscle, Pancreas, Placenta, Prostate, Spleen, Testis tissues are correlated with this protein. The following patways have been known to be associated with this gene.