PLAU elisa kit product blog
Tags: ELISA Kit; PLAU; PLAU elisa kit; uPA functional assay;
The General PLAU plau (Catalog #MBS135509) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS135509 ELISA Kit recognizes General PLAU.The PLAU plau product has the following accession number(s) (GI #222537759) (NCBI Accession #NP_001138503.1) (Uniprot Accession #P00749). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Active human uPA functional assay ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Intended Uses: Human uPA activity assay is intended for the quantitative determination of active urokinase plasminogen activator in human plasma. For research use only.
Principle of the Assay: Functionally active uPA present in plasma will form a covalent complex with the biotinylated human PAI-1 which is bound to the avidin on the plate. Latent or complexed uPA will not bind to the plate and will not be detected. Unbound uPA samples are washed away and an anti-uPA primary antibody is added. Excess primary antibody is washed away and bound antibody is then reacted with the horseradish peroxidase secondary antibody. Following an additional washing step, TMB is then used for color development at 450nm. The amount of color development is directly proportional to the concentration of active uPA in the sample.
Background: Urokinase plasminogen activator, along with its receptor uPAR, initiates a proteolytic cascade that results in the conversion of plasminogen to plasmin [1]. Clinical studies have indicated that high uPA levels may elevate the risk for tumor invasion and metastasis [2,4]. Increased expression and activity can exert potent arthritogenic properties in rheumatoid arthritis patients [3]. Increased uPA activity may be an implication for the pathophysiology of endometriosis [5].
Samples: Human plasma
Sensitivity: The minimum detectable dose (MDD) was determined by adding two standard deviations to the mean optical density value of twenty-two zero standard replicates (range OD450: 0.05-0.055) and calculating the corresponding concentration. The MDD was 0.015 ng/ml. Intra-assay Precision: These studies are currently in progress. Please contact us for more information.
Inter-assay Precision: These studies are currently in progress. Please contact us for more information. Active human uPA functional assay ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.