PL (IgG) elisa kit product blog
Tags: ELISA Kit; Human ELISA Kit; anti-phospholipid (PL) antibody (IgG); PL (IgG) elisa kit; PL (IgG);
The Human PL (IgG) n/a (Catalog #MBS9310804) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS9310804 ELISA Kit recognizes Human PL (IgG).To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Principle of the assay: The kit uses a double-antigen sandwich enzyme-linked immunosorbent assay (ELISA) to analyze the existence or not of Human PL-IgG in samples. Add sample to wells pre-coated with one Human Phospholipid Antibody IgG antigen, at same time add HRP-conjugated Human Phospholipid Antibody IgG antigen to bind the analyte, followed by incubation and washing procedures to remove unbound substance. Finally, HRP substrates are added, incubated for detection, and a blue color is developed. Reaction is stopped and color turns to yellow when Stopping Solution (acidic) is added. The existence or not of Human PL-IgG in the samples is then determined by comparing the O.D. of the samples to the CUT OFF.
Samples: Serum, plasma, tissue homogenate, cell culture supernates and other biological fluids
Assay Type: Sandwich. Intra-assay Precision: Intra-assay CV (%) are less than 15%.
Inter-assay: Inter-assay CV (?) are less than 15%.
Intended Uses: For the qualitative determination of activated Human Phospholipid Antibody IgG (PL-IgG) in serum, plasma, tissue homogenate, cell culture supernates and other biological fluids. Human anti-phospholipid (PL) antibody (IgG) ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.