Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
Fax 1.858.633.0166    Email 
menu1 Home Products Ordering Company Help menu7
   MyBioSource  rightarrow Assay Kit Blog  rightarrow PKIG elisa kit  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Blogs moreseparator
 • CNP elisa kit blog
 • EGFR elisa kit blog
 • Casp7 elisa kit blog
 • BCL-2 elisa kit blog
 • CTACK elisa kit blog
 • GRM6 elisa kit blog
 • TGFbeta3 elisa kit blog
 • SDC1/CD138 elisa kit blog
 • hsa-let-7b Real-time RT-PCR pcr kit blog
 • MAOB elisa kit blog
 • BIRC7 elisa kit blog
 • EFNA3 elisa kit blog
 • PRNP elisa kit blog
 • FGF15 elisa kit blog
 • GPX3 elisa kit blog
 • PVALB elisa kit blog
 • HB-EGF elisa kit blog
 • ERCC3 elisa kit blog
 • CCND1 elisa kit blog
 • AKAP13 elisa kit blog
Blog Categories separator
 • Assay Kit Blogs
 • Biochemical Blogs
 • cDNA Clone Blogs
 • Monoclonal Antibody Blogs
 • Polyclonal Antibody Blogs
 • Rec/Purified Protein Blogs
 • Popular Product Blogs
Our Best Sellers moreseparator
 • MD-2 (LY96) Antibody
 • Interferon alpha (IFN-alpha) ELISA Kit
 • Transferrin Receptor (TFR) ELISA Kit
 • Testosterone ELISA Kit
 • Vimentin (VIM) Recombinant Protein
 • Goat Anti-Human IgG, Monkey ads Secondary Antibody
 • brain-derived neurotrophic factor (BDNF) Antibody
 • 4E-BP1 Antibody
 • Interleukin-6 protein (Il6) Recombinant Protein
 • Calcium-Calmodulin Dependent Protein Kinase Kinase 2 (CAMKK2... ELISA Kit
 • TAP2 Antibody
 • Thyroglobulin (TG) Antibody
 • UHRF1-binding protein 1-like (UHRF1BP1L) Recombinant Protein
 downarrow more ...

PKIG elisa kit product blog

Posted on 2015-09-26 09:07:06 by mybiosource_staff
Share it on ...     
Tags: ELISA Kit; Rabbit ELISA Kit; PKIG; PKIg elisa kit; cAMP-dependent protein kinase inhibitor gamma (PKIG);
The Rabbit PKIG pkig (Catalog #MBS7209634) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS7209634 ELISA Kit recognizes Rabbit (General) PKIG.

The PKIG pkig product has the following accession number(s) (GI #527122104) (NCBI Accession #NP_001268374.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

(Or you can also download the PDF Manual for complete product instructions).

Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Rabbit cAMP-dependent protein kinase inhibitor gamma (PKIG) ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only) PKIG.

Intended Uses: This PKIG ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rabbit PKIG. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: PKIG ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PKIG antibody and an PKIG-HRP conjugate. The assay sample and buffer are incubated together with PKIG-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PKIG concentration since PKIG from samples and PKIG-HRP conjugate compete for the anti-PKIG antibody binding site. Since the number of sites is limited, as more sites are occupied by PKIG from the sample, fewer sites are left to bind PKIG-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PKIG concentration in each sample is interpolated from this standard curve.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type: Competitive (Quantitative)
Sensitivity: 0.1 ng/mL. Rabbit cAMP-dependent protein kinase inhibitor gamma (PKIG) ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. Blood, Bone, Brain, Heart, Kidney, Liver, Muscle, Pancreas, Parathyroid, Skin tissues are correlated with this protein.
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions