Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource  rightarrow Assay Kit Blog  rightarrow PKCa elisa kit  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Blogs moreseparator
separator
 • TSP-1 elisa kit blog
 • CPN2 elisa kit blog
 • HDL-C elisa kit blog
 • OH-PYD elisa kit blog
 • MRP elisa kit blog
 • WDR35 elisa kit blog
 • TREML2 elisa kit blog
 • alpha Actin elisa kit blog
 • CSN3 elisa kit blog
 • RPL27 elisa kit blog
 • TRPV1 elisa kit blog
 • Cys-C elisa kit blog
 • C12orf36 elisa kit blog
 • RNLS elisa kit blog
 • ADH elisa kit blog
 • ZBTB20 elisa kit blog
 • N-AChTR elisa kit blog
 • HDGF elisa kit blog
 • IFN-gamma elisa kit blog
 • STAT5B elisa kit blog
separator
Blog Categories separator
separator
 • Assay Kit Blogs
 • Biochemical Blogs
 • cDNA Clone Blogs
 • Monoclonal Antibody Blogs
 • Polyclonal Antibody Blogs
 • Rec/Purified Protein Blogs
 • Popular Product Blogs
separator
Our Best Sellers moreseparator
separator
 • Gamma Glutamyl Transpeptidase (GGT) ELISA Kit
 • CDCP1 Antibody
 • SLC17A2 Antibody
 • YWHAE Antibody
 • MIBP1 Antibody
 • RIOK2 Antibody
 • KCNC1 Antibody
 downarrow more ...
separator

PKCa elisa kit product blog

separator
Posted on 2015-11-01 10:20:10 by mybiosource_staff
Share it on ...     
separator
separator
Tags: ELISA Kit; Rat ELISA Kit; PKCA elisa kit; PKCA; Protein Kinase C Alpha (PKCa);
separator
The Rat PKCa prkca (Catalog #MBS2022832) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS2022832 ELISA Kit recognizes Rat (Rattus, General) PKCa.

The PKCa prkca product has the following accession number(s) (GI #15099971) (NCBI Accession #AAK84184.1) (Uniprot Accession #P17252). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


(Or you can also download the PDF Manual for complete product instructions).

Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Protein Kinase C Alpha (PKCa) ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only) PKCa.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Kinase C Alpha (PKCa). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein Kinase C Alpha (PKCa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Kinase C Alpha (PKCa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protein Kinase C Alpha (PKCa) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Type: Double-antibody Sandwich
Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
Detection Range: 0.312-20ng/mL
Sensitivity: < 0.117ng/mL. Application: Enzyme-linked immunosorbent assay for Antigen Detection.
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Protein Kinase C Alpha (PKCa) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Protein Kinase C Alpha (PKCa) were tested on 3 different plates, 8 replicates in each plate.
CV(%) =: SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%. Protein Kinase C Alpha (PKCa) ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. The following patways have been known to be associated with this gene. Brain Diseases, Breast Neoplasms, Cardiovascular Diseases, Disease Models, Animal, Heart Diseases, Hypertension, Inflammation, Necrosis, Neoplasms, Experimental, Nervous System Diseases are some of the diseases may be linked to Protein Kinase C Alpha (PKCa) ELISA Kit. Blood, Brain, Eye, Heart, Intestine, Kidney, Liver, Lung, Muscle, Skin tissues are correlated with this protein. PKCa also interacts with the following gene(s): EZR, GJA1, MARCKS, PICK1, PLD1, PLD2, RAC1, RARA, RICTOR, SDC4.
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions