|The Human PKB ptk2b (Catalog #MBS014592) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS014592 ELISA Kit recognizes Human (General) PKB.
The PKB ptk2b product has the following accession number(s) (GI #27886588) (NCBI Accession #NP_775267.1) (Uniprot Accession #Q14289). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
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Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Human Protein Kinase B ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Introduction: ELISA is a simple and highly sensitive method of analysis that allows for simultaneous and rapid quantification of a large number of samples. The assay is based on the specific recognition of the target compound (analyte/antigen) by antibodies which bind to the compound. The antigen-antibody complex is detected and measured with the aid of an enzyme-labeled antibody or antigen. Upon addition of a non-colored reagent, the enzyme produces a color reaction where the color intensity is directly or inversely proportional to the concentration of the analyte in the sample.
Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids
Detection Range: 25 U/L - 800 U/L.
Sensitivity: 2.0 U/L. Intended Uses: This ELISA kit is intended for laboratory in vitro research use only, not for drug, household or other use! The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm by a spectrophotometer. In order to measure the concentration of Human PKB in the sample, this ELISA kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus Human PKB concentration. The concentration of Human PKB in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Intra-assay Precision: Intra-assay CV (%) are less than 15%.
Inter-assay Precision: Inter-assay CV (%) are less than 15%. Human Protein Kinase B ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. Blood, Bone Marrow, Brain, Embryonic Tissue, Lymph, Lymph Node, Muscle, Testis tissues are correlated with this protein.