|The Human PIP5K1A pip5k1a (Catalog #MBS281793) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS281793 ELISA Kit recognizes Human (General) PIP5K1A.
The PIP5K1A pip5k1a product has the following accession number(s) (GI #208431778) (NCBI Accession #NP_001129110.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
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Intended Uses: For the quantitative detection of Human Phosphatidylinositol-4-phosphate 5-kinase type-1 alpha (PIP5K1A) concentration in serum, plasma and other biological fluids.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitative PIP5K1A in Human serum, plasma. An antibody specific for PIP5K1A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PIP5K1A present is bound by the immobilized antibody. After removing any unbound substances, a biotin - conjugated antibody specific for PIP5K1A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PIP5K1A bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Phosphatidylinositol-4-phosphate 5-kinase type-1 alpha (PIP5K1A) ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. The following patways have been known to be associated with this gene. Brain, Liver, Testis tissues are correlated with this protein. PIP5K1A also interacts with the following gene(s): PI4KB.