PIK3CA elisa kit product blog
Tags: ELISA Kit; Human ELISA Kit; PIK3CA; PIK3CA elisa kit;
The Human PIK3CA n/a (Catalog #MBS846735) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS846735 ELISA Kit recognizes Human PIK3CA.To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the PIK3CA (Human) ELISA Kit with the following immunoassay(s):
Typical Standard Curve/Testing Data
Background/Introduction: Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns (Phosphatidylinositol), PtdIns4P (Phosphatidylinositol 4-phosphate) and PtdIns(4,5)P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Involved in the activation of AKT1 upon stimulation by receptor tyrosine kinases ligands such as EGF, insulin, IGF1, VEGFA and PDGF. Essential in endothelial cell migration during vascular development through VEGFA signaling, possibly by regulating RhoA activity. Participates in cardiomyogenesis in embryonic stem cells through an AKT1 pathway. Participates in vasculogenesis in embryonic stem cells through PDK1 and protein kinase C pathway. PIK3CA ELISA kit is a sandwich ELISA assay for the quantitative measurement of human PIK3CA in serum, plasma and cell culture supernatants. The density of color is proportional to the amount of human PIK3CA captured from the samples.
Samples: Serum, plasma and cell culture supernatants
Assay Type: Quantitative Sandwich
Detection Range: 0.156-10 ng/ml
Sensitivity: < 0.094 ng/ml. PIK3CA (Human) ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.