PI Ab -IgG/IgM elisa kit product blog
Tags: ELISA Kit; Rabbit ELISA Kit; Phosphatidylinositol Antibody IgG/IgM; PI Ab -IgG/IgM elisa kit; PI Ab -IgG/IgM;
The Rabbit PI Ab -IgG/IgM n/a (Catalog #MBS730263) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS730263 ELISA Kit recognizes Rabbit PI Ab -IgG/IgM.To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Rabbit Phosphatidylinositol antibody IgG/IgM ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: PI Ab -IgG/IgM ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with Phosphatidylinositol antigen IgG/IgM. Standards or samples are then added to the microtiter plate wells and PI Ab -IgG/IgM if present, will bind to the antigen pre-coated wells. In order to quantitatively determine the amount of PI Ab -IgG/IgM present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated Phosphatidylinositol antigen IgG/IgM are added to each well to "sandwich" the PI Ab -IgG/IgM immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain PI Ab -IgG/IgM and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PI Ab -IgG/IgM concentration in each sample is interpolated from this standard curve.
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type: Competitive or Sandwich
Detection Range: 10-250ng/mL
Sensitivity: 1.0ng/mL. Intended Uses: This PI Ab -IgG/IgM ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rabbit PI Ab -IgG/IgM. This ELISA kit for research use only, not for therapeutic applications!. Rabbit Phosphatidylinositol antibody IgG/IgM ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.