|The Most species Phosphatidic Acid (PA) n/a (Catalog #MBS2000130) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS2000130 ELISA Kit recognizes Most species Phosphatidic Acid (PA).
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual
for complete product instructions).
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phosphatidic Acid (PA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Phosphatidic Acid (PA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phosphatidic Acid (PA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Phosphatidic Acid (PA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Samples: Tissue or cell culture supernates
Assay Type: Competitive
Detection Range: 1.23-100ng/mL
Sensitivity: Typically less than 0.45ng/mL. Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Phosphatidic Acid (PA) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Phosphatidic Acid (PA) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
. Phosphatidic Acid (PA) ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.