|The Most species Phosphatidic Acid (PA) n/a (Catalog #MBS2000130) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS2000130 ELISA Kit recognizes Most species Phosphatidic Acid (PA).
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for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Phosphatidic Acid (PA) ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of PA in cell lysates and other biological fluids.
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to PA has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled PA and unlabeled PA (Standards or samples) with the pre-coated antibody specific to PA. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of PA in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of PA in the sample.
Samples: Cell lysates and other biological fluids
Assay Type: Quantitative Competitive
Detection Range: 1.23-100ng/mL
Sensitivity: 0.45ng/mL. Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level PA were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level PA were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%. Phosphatidic Acid (PA) ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.