pGSK-3 elisa kit product blog
Tags: ELISA Kit; Human ELISA Kit; Phospho glycogen synthase kinase 3; pGSK-3 elisa kit; pGSK-3;
The Human pGSK-3 n/a (Catalog #MBS281629) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS281629 ELISA Kit recognizes Human pGSK-3.To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Intended Uses: For the quantitative detection of Human Phospho glycogen synthase kinase 3 (pGSK-3) concentration in serum, plasma and other biological fluids.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate pGSK-3 in Human serum, plasma. An antibody specific for pGSK-3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any pGSK-3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for pGSK-3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of pGSK-3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Samples: Serum, plasma and other biological fluids
Assay Type: Quantitative Sandwich
Detection Range: 62.5 pg/mL - 4000 pg/mL
Sensitivity: 31.25 pg/mL (mean of 6 independent assays). Intra-assay Precision: Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Intra-Assay: CV<8%
Inter-assay Precision: Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100. Inter-Assay: CV<12%. Human Phospho glycogen synthase kinase 3 (pGSK-3) ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.