Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
Fax 1.858.633.0166    Email 
menu1 Home Products Ordering Company Help menu7
   MyBioSource  rightarrow Assay Kit Blog  rightarrow PFDN5 elisa kit  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Blogs moreseparator
 • IFABP elisa kit blog
 • PR elisa kit blog
 • MTRF1 elisa kit blog
 • CELSR2 elisa kit blog
 • PLXNB1 elisa kit blog
 • ETFDH elisa kit blog
 • AAP clia kit blog
 • PR3AB elisa kit blog
 • KERA elisa kit blog
 • 5-LOX elisa kit blog
 • HSPD1 elisa kit blog
 • Bcl2 elisa kit blog
 • KHK elisa kit blog
 • TXA2S elisa kit blog
 • mmu-mir-10a pcr kit blog
 • CBLC elisa kit blog
 • JAKMIP1 elisa kit blog
 • PON2 elisa kit blog
 • CABLES1 elisa kit blog
 • hnRNP / RA33 elisa kit blog
Blog Categories separator
 • Assay Kit Blogs
 • Biochemical Blogs
 • cDNA Clone Blogs
 • Monoclonal Antibody Blogs
 • Polyclonal Antibody Blogs
 • Rec/Purified Protein Blogs
 • Popular Product Blogs
Our Best Sellers moreseparator
 • ZNF449 cDNA Clone
 • Testosterone ELISA Kit
 • Interleukin-1 receptor (IL1R1) Antibody
 • Retinoic acid receptor responder protein 2 (RARRES2) Recombinant Protein
 • DUSP1 Antibody
 • Marijuana (THC) Antibody
 • interleukin 19 (IL19) ELISA Kit
 • Desacyl Ghrelin (D-GHR) ELISA Kit
 • PLAP Antibody
 • NAP1L1 Antibody
 • 6-pyruvoyltetrahydropterin synthase (PTS) Antibody
 • DDB1 Antibody
 • Cyclin-B1-1 (CYCB1-1) Recombinant Protein
 downarrow more ...

PFDN5 elisa kit product blog

Posted on 2016-09-18 16:15:41 by mybiosource_staff
Share it on ...     
Tags: ELISA Kit; Mouse ELISA Kit; PFDN5; Prefoldin subunit 5 (PFDN5); PFDN5 elisa kit;
The Mouse PFDN5 pfdn5 (Catalog #MBS7218002) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS7218002 ELISA Kit recognizes Mouse (Murine, General) PFDN5.

The PFDN5 pfdn5 product has the following accession number(s) (GI #27807413) (NCBI Accession #NP_777157.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

(Or you can also download the PDF Manual for complete product instructions).

Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Mouse Prefoldin subunit 5 (PFDN5) ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only) PFDN5.

Intended Uses: This PFDN5 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse PFDN5. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: PFDN5 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PFDN5 antibody and an PFDN5-HRP conjugate. The assay sample and buffer are incubated together with PFDN5-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PFDN5 concentration since PFDN5 from samples and PFDN5-HRP conjugate compete for the anti-PFDN5 antibody binding site. Since the number of sites is limited, as more sites are occupied by PFDN5 from the sample, fewer sites are left to bind PFDN5-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PFDN5 concentration in each sample is interpolated from this standard curve.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type: Competitive
Detection Range: 0.5-10ng/mL
Sensitivity: 0.1ng/mL. Mouse Prefoldin subunit 5 (PFDN5) ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. Brain, Kidney, Liver, Lung, Muscle, Pancreas tissues are correlated with this protein.
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions