PC4 clia kit product blog
Tags: CLIA Kit; PC4; Positive Cofactor 4 (PC4); PC4 clia kit;
The PC4 n/a (Catalog #MBS2000471) is a CLIA Kit and is intended for research purposes only. The product is available for immediate purchase.To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given clia kit. We\'ve tested the CLIA Kit for Positive Cofactor 4 (PC4) with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated secondary antibody. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate An and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Positive Cofactor 4 (PC4) level in the sample or standard.
Assay Type: Sandwich
Samples: Tissue homogenates and other biological fluids. Detection Range: 6.859-5000pg/mL The standard curve concentrations used for the ELISA\'s were 5000pg/mL, 1666.67pg/mL, 555.56pg/mL, 185.19pg/mL, 61.728pg/mL, 20.576pg/mL, 6.859pg/mL
Sensitivity: The minimum detectable dose of this kit is typically less than 33pg/mL
Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Positive Cofactor 4 (PC4) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Positive Cofactor 4 (PC4) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Assay Procedure Summary1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37oC;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37oC;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37oC;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37oC;
8. Read RLU value immediately.
Organism Species: Homo sapiens (Human)
Assay Length: 4.5 hours.