Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
Fax 1.858.633.0166    Email 
menu1 Home Products Ordering Company Help menu7
   MyBioSource  rightarrow Assay Kit Blog  rightarrow PAI-2 elisa kit  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Blogs moreseparator
 • OVA sIgG1 elisa kit blog
 • DEFB3 elisa kit blog
 • SV2C elisa kit blog
 • RPS29 elisa kit blog
 • HIS elisa kit blog
 • ALK1 elisa kit blog
 • CCL2 elisa kit blog
 • RPL13A elisa kit blog
 • CST6 elisa kit blog
 • BEND6 elisa kit blog
 • BCAP31 elisa kit blog
 • RIC8A elisa kit blog
 • CKMT1A elisa kit blog
 • GRINA elisa kit blog
 • AA elisa kit blog
 • ACTalpha1 elisa kit blog
 • GAP elisa kit blog
 • PDRG1 elisa kit blog
 • SLC30A5 elisa kit blog
 • CTSE elisa kit blog
Blog Categories separator
 • Assay Kit Blogs
 • Biochemical Blogs
 • cDNA Clone Blogs
 • Monoclonal Antibody Blogs
 • Polyclonal Antibody Blogs
 • Rec/Purified Protein Blogs
 • Popular Product Blogs
Our Best Sellers moreseparator
 • chromosome 6 open reading frame 105 (C6orf105) ELISA Kit
 • PAPD5 Antibody
 • FCNA Antibody
 • Alpha-CGRP (8-37) Peptide
 • Insulin Like Growth Factor 1 (IGF-1) ELISA Kit
 • Kalirin (KALRN) Antibody
 • Phosphatidylethanolamine Binding Protein 1 (PEBP1) ELISA Kit
 • Enterovirus Pan-reactive VP3 Antibody
 • carcinoembryonic antign, CEA ELISA Kit
 • Calcitonin Gene Related Peptide (CGRP) ELISA Kit
 • phenylalanyl-tRNA synthetase, alpha subunit (FARSA) ELISA Kit
 • UBE2O Antibody
 • IL-12 P40 ELISA Kit
 • MAPT-T212 Antibody
 downarrow more ...

PAI-2 elisa kit product blog

Posted on 2016-07-21 12:18:47 by mybiosource_staff
Share it on ...     
Tags: ELISA Kit; Mouse ELISA Kit; Plasminogen Activator Inhibitor 2; PAI-2; PAI-2 elisa kit;
The Mouse PAI-2 serpinb2 (Catalog #MBS761904) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS761904 ELISA Kit recognizes Mouse (Murine, General) PAI-2.

The PAI-2 serpinb2 product has the following accession number(s) (GI #219689110) (NCBI Accession #NP_001137290.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

(Or you can also download the PDF Manual for complete product instructions).

Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Mouse Plasminogen Activator Inhibitor 2 ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only) PAI-2.

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- PAI-2 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- PAI-2 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the PAI-2 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of PAI-2 can be calculated.

Samples: Serum, plasma and other biological fluids.
Assay Type: Sandwich ELISA, Double Antibody
Detection Range: 0.391-25ng/ml
Sensitivity: 0.234ng/ml. Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level PAI- 2 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level PAI- 2 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%. Mouse Plasminogen Activator Inhibitor 2 ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. Breast Neoplasms, Carcinoma, Carcinoma, Squamous Cell, Cardiovascular Diseases, Hypertension, Inflammation, Lung Diseases, Necrosis, Neoplasm Invasiveness, Skin Neoplasms are some of the diseases may be linked to Mouse Plasminogen Activator Inhibitor 2 ELISA Kit. Blood, Brain, Embryonic Tissue, Heart, Lung, Mouth, Muscle, Skin, Uterus, Vascular tissues are correlated with this protein. The following patways have been known to be associated with this gene. PAI-2 also interacts with the following gene(s): CLCA1, PLAU, PLAUR, RB1, TLR4.
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions