Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource  rightarrow Assay Kit Blog  rightarrow PAI-1 elisa kit  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Blogs moreseparator
separator
 • GLDC elisa kit blog
 • CTAG2 elisa kit blog
 • PKIG elisa kit blog
 • CDKN3 elisa kit blog
 • S17AH elisa kit blog
 • RIMBP2 elisa kit blog
 • CDC elisa kit blog
 • ABCD3 elisa kit blog
 • MRC2 elisa kit blog
 • TNC elisa kit blog
 • VEGFA elisa kit blog
 • anti-sperm antibody, AsAb elisa kit blog
 • CAMK2 elisa kit blog
 • DNMT3A elisa kit blog
 • KLK2 elisa kit blog
 • DDIT4L elisa kit blog
 • Bax elisa kit blog
 • PRNP elisa kit blog
 • CAPN6 elisa kit blog
 • TTC21A elisa kit blog
separator
Blog Categories separator
separator
 • Assay Kit Blogs
 • Biochemical Blogs
 • cDNA Clone Blogs
 • Monoclonal Antibody Blogs
 • Polyclonal Antibody Blogs
 • Rec/Purified Protein Blogs
 • Popular Product Blogs
separator
Our Best Sellers moreseparator
separator
 • 25-Dihydroxy Vitamin D3 (25-DVD3) ELISA Kit
 • IRTKS Antibody
 • 5-hydroxytryptamine (serotonin) receptor 6 (HTR6) ELISA Kit
 • NFkB, p65 (NKIRAS2) Antibody
 • NMDA NR1 Subunit (GRIN1) Antibody
 • PSMC4 Antibody
 • Actinin Alpha 2 (ACTN2) ELISA Kit
 • Advanced oxidation protein products (AOPP) ELISA Kit
 • Steroidogenic factor 1 (NR5A1) Recombinant Protein
 • Voltage-gated potassium channel (VGKC) ELISA Kit
 • Kappa Light Chain Antibody
 • 1,2-dihydroxy-3-keto-5-meth ylthiopentene dioxygenase 1 (ADI1... Recombinant Protein
 • HIV-1 p24 Recombinant Protein
 • Serine-threonine-protein kinase-endoribonuclease IRE1 (ERN1)... Recombinant Protein
 downarrow more ...
separator

PAI-1 elisa kit product blog

separator
Posted on 2016-08-18 08:49:28 by mybiosource_staff
Share it on ...     
separator
separator
Tags: ELISA Kit; PAI-1; PAI-1 elisa kit;
separator
The PAI-1 serpine1 (Catalog #MBS136003) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase.

The PAI-1 serpine1 product has the following accession number(s) (GI #10835159) (NCBI Accession #NP_000593.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


(Or you can also download the PDF Manual for complete product instructions).

Plasminogen activator inhibitor type 1 (PAI-1) is involved in the regulation of the blood fibrinolytic system. Increased plasma levels of PAI-1 are implicated in the impairment of fibrinolytic function and may be associated with thrombotic diseases. Levels of PAI-1 increase with age and are elevated in conditions such as normal pregnancy and sepsis. The sensitive quantitative measurement of functionally active human PAI-1 in non-plasma samples including cell culture media or tissue extracts is easily performed with this 96 well strip format ELISA kit. The assay measures active PAI-1 in the 0.125-100 ng/ml range. Samples giving human PAI-1 levels above 100 ng/ml should be diluted in blocking buffer before use. Functionally active PAI-1 reacts with urokinase coated onto a micro titer plate. Latent or complexed PAI-1 will not bind to the plate and will not be detected by the assay. Vitronectin does not interfere with the detection of active PAI-1. This kit does not cross react with mouse PAI-1. After appropriate washing steps, anti human PAI-1 primary antibody binds to the PAI-1. Excess antibody is washed away, and bound antibody is reacted with the secondary antibody conjugated to HRP. Following an additional washing step, TMB substrate is used for color development at 450 nm. Color development is proportional to the concentration of PAI-1 in the samples. A standard calibration curve is prepared in blocking buffer using dilutions of purified PAI-1 and is measured along with the test samples. All reagents and standards are provided in these ELISA kits. This assay uses an exclusive enzyme capture technology to only detect functionally active protein.

PAI-1 serpine1

Active Human PAI-1 Functional Assay ELISA Kit for Non Plasma Samples or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. Adipose Tissue, Blood, Brain, Connective Tissue, Heart, Kidney, Liver, Lung, Muscle, Vascular tissues are correlated with this protein. Atherosclerosis, Cardiovascular Diseases, Fibrosis, Heart Diseases, Hypertension, Inflammation, Necrosis, Nervous System Diseases, Obesity, Thrombosis are some of the diseases may be linked to Active Human PAI-1 Functional Assay ELISA Kit for Non Plasma Samples.
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions