PA-IgM elisa kit product blog
Tags: ELISA Kit; Human ELISA Kit; PA-IgM elisa kit; PA-IgM; Platelet IgM;
The Human PA-IgM n/a (Catalog #MBS282819) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS282819 ELISA Kit recognizes Human PA-IgM.To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Principle of the Assay: This assay employs the competitive enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to PA-IgM. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated PA-IgM and incubated. The competitive inhibition reaction is launched between with HRP labeled PA-IgM and unlabeled PA-IgM with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of PA-IgM in the sample. The color development is stopped and the intensity of the color is measured.
Samples: Serum, Plasma, Other biological fluids
Assay Type: Competitive ELISA. Sample Volume: 1-200 uL
Precision: Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength: 450 nm. Human Platelet IgM antibodies (PA-IgM) ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.