|The OxiSelect TBARS n/a (Catalog #MBS168658) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase.
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Please refer to the product datasheet for known applications of a given assay kit. We\'ve tested the OxiSelect TBARS Assay Kit (MDA Quantitation) with the following immunoassay(s):
Testing Data #2
Principle of the assay: The Thiobarbituric Acid Reactive Substances (TBARS) Assay Kit is a tool for the direct quantitative measurement of MDA in biological samples. The unknown MDA containing samples or MDA standards are first reacted with TBA at 95 degree C. After a brief incubation, the samples and standards can be read either spectrophotometrically or fluorometrically. The MDA content in unknown samples is determined by comparison with the predetermined MDA standard curve.
Background: Lipid peroxidation is a well-defined mechanism of cellular damage in animals and plants. Lipid peroxides are unstable indicators of oxidative stress in cells that decompose to form more complex and reactive compounds such as Malondialdehyde (MDA) and 4-hydroxynonenal (4-HNE), natural bi-products of lipid peroxidation. Oxidative modification of lipids can be induced in vitro by a wide array of pro-oxidant agents and occurs in vivo during aging and in certain disease conditions. Measuring the end products of lipid peroxidation is one of the most widely accepted assays for oxidative damage. These aldehydic secondary products of lipid peroxidation are generally accepted markers of oxidative stress. Thiobarbituric Acid Reactive Substances (TBARS) is a well-established assay for screening and monitoring lipid peroxidation. The rapid and easy protocol has been modified by researchers in the evaluation of drugs, food, as well as human and animal tissue samples. MDA forms a 1:2 adduct with thiobarbituric acid (Figure 1). The MDA-TBA adduct formed from the reaction of MDA in samples with TBA can be measured colorimetrically or fluorometrically. TBARS levels are determined from a Malondialdehyde equivalence standard. The TBARS Assay has provided relevant information concerning free radical activity in disease states and measurement of many compounds anti-oxidant characteristics. Although the specificity of TBARS toward compounds other than MDA has been controversial, the assay continues to be the most widely employed format for monitoring lipid peroxidation. Lipids with higher degrees of unsaturated bonds produce higher TBARS values. Interfering soluble TBARS can be minimized if lipoprotein fractions are first acid precipitated from samples. Biological samples may contain a mixture of thiobarbituric acid reactive substances such as hydroperoxides and aldehydes, which increase in response to oxidative stress. If excessively high TBARS values are obtained, a more specific assay such as HPLC should be employed. The OxiSelect TBARS Assay Kit offers a simple, reproducible, and consistent system for the detection of lipid peroxidation in urine, plasma, serum, lysates, and tissue homogenates. This kit includes an MDA standard for use as a positive control. Each kit provides sufficient reagents to perform 200 tests including standard curve and unknown samples.