OxiSelect Myleoperoxidase Chlorination Activity assay kit product blog
Tags: Assay Kit; OxiSelect Myleoperoxidase Chlorination Activity; OxiSelect Myleoperoxidase Chlorination Activity assay kit;
The OxiSelect Myleoperoxidase Chlorination Activity n/a (Catalog #MBS168265) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase.To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given assay kit. We\'ve tested the OxiSelect Myleoperoxidase Chlorination Activity Assay Kit (Fluorometric) with the following immunoassay(s):
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Principle of the assay: The OxiSelect Myeloperoxidase Chlorination Activity Assay Kit is a quantitative fluorescence-based assay for measuring the myeloperoxidase chlorination activity within a sample. Ferric, or native, MPO enzyme reacts with hydrogen peroxide (H2O2) to form the active redox intermediate MPO-I. The MPO-I then oxidizes chloride ions (Cl-) to create hypochlorous acid (HOCl). This reaction encompasses the chlorination cycle. Hypochlorite ion (OCl-) then cleaves the non-fluorescent aminophenyl fluorescein (APF) to generate fluorescein (lambdaex = 485nm/lambdaem = 525nm). The myeloperoxidase activity in unknown samples is determined by comparison with the predetermined fluorescein standard curve.
Background: Myeloperoxidase (MPO) is a heme-based peroxidase enzyme responsible for antimicrobial activity against a wide range of organisms. MPO is found in neutrophils, monocytes, and some soft tissue macrophages. MPO is copiously expressed in stimulated neutrophil granulocytes, where it catalyzes the production of hypoalous acids, such as hypochlorous acid (HOCl), from hydrogen peroxide (H2O2) and chloride ion (Cl-), or other halides. The characteristic heme green pigment of MPO can be seen in secretions containing neutrophils such as pus and mucus. Myeloperoxidase has been implicated in many disease states including arthritis, cancer, renal dysfunction, and cystic fibrosis. While myeloperoxidase deficiency is a hereditary disease that results in immune deficiency, antibodies against MPO have been linked to various types of vasculitis. The reactive species it creates may also damage normal tissues, thereby contributing to inflammation. An association of elevated MPO levels and coronary artery disease has been demonstrated and it has been reported that the enzyme could serve as a predictor of myocardial infarction in certain patients. The numerous roles MPO plays in the innate immune response and disease states make it a target for potential therapies. MPO exhibits both chlorination and peroxidation activities (see Figure 1). OxiSelect Myeloperoxidase Chlorination Activity Assay Kit is a quantitative fluorescence assay for measuring myeloperoxidase activity within cell lysates and purified enzyme samples. The assay is not applicable for use in serum samples. Myeloperoxidase activity is defined as follows: 1 unit of MPO enzyme is the amount of enzyme that will oxidize the MPO substrate to generate 1 mumole of fluorescein per minute at 25 degree C. Each kit provides sufficient reagents to perform up to 192 assays, including standard curve and unknown samples.