OxiSelect AOPP assay kit product blog
Tags: Assay Kit; OxiSelect AOPP; OxiSelect AOPP assay kit;
The OxiSelect AOPP n/a (Catalog #MBS168459) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase.To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given assay kit. We\'ve tested the OxiSelect AOPP Assay Kit (200 assays) with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Principle of the assay: The Advanced Oxidation Protein Products (AOPP) Assay Kit is a bioassay tool for the direct quantitative measurement of AOPPs in biological samples. The unknown AOPP-containing samples or Chloramine standards are first mixed with an assay reaction initiator that begins a color development process. After a brief incubation, a stop solution is added and the samples and standards can be read with a standard colorimetric plate reader. The AOPP content in unknown samples is determined by comparison with the predetermined Chloramine standard curve.
Background: Oxidative stress is defined as an increase in the production of reactive oxygen species (ROS) due to an imbalance between antioxidant and oxidants. Advanced Oxidation Protein Products (AOPP) are uremic toxins created during oxidative stress through the reaction of chlorinated oxidants, such as chloramines and hypochlorous acid, with plasma proteins. AOPPs are structurally similar to Advanced Glycation End-Product (AGE) proteins and exert similar biological activities. AOPPs are elevated in patients with renal complications, atherosclerosis, diabetes mellitus, systemic sclerosis, as well as HIV-positive patients. Human Serum Albumin (HSA) treated with HOCl and AOPP generated in vivo can ignite oxidative reactions in both neutrophils and monocytes, which indicates both can be used as true mediators of inflammation. Although the mechanisms of AOPP degradation and elimination from the blood remain to be fully elucidated, it appears that the liver and spleen are mostly responsible for their isolation and removal. The AOPP Assay has provided relevant information concerning free radical activity in many uremic associated disease states and the measurement of anti-oxidant characteristics of many compounds. The rapid and easy protocol has been modified by researchers in the evaluation of plasma and tissue samples. The AOPP-HSA concentration was determined from a Chloramine equivalence standard. The OxiSelect AOPP Assay Kit offers a simple, reproducible, and consistent system for the detection of advanced oxidation protein products in plasma, lysates, and tissue homogenates. This kit includes a Chloramine standard and an AOPP Human Serum Albumin conjugate for use as a positive control. Each kit provides sufficient reagents to perform 200 tests including standard curve and unknown samples.