|The Oxaloacetate n/a (Catalog #MBS841520) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase.
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Please refer to the product datasheet for known applications of a given assay kit. We\'ve tested the Oxaloacetate Colorimetric / Fluorometric Assay Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Product Information: Oxaloacetate Assay Kit: Colorimetric & Fluorometric Assay for Quantifying Oxaloacetate (OAA) in a variety of Samples such as Tissues, Cells etc. within 40 min. Rapid, Convenient & Reliable. Detection Range: 0.1-10nmol (2-200 muM). 100 Assays.
Background: Oxaloacetate (OAA, HOOC-CO-CH?-COOH) is a TCA cycle intermediate. It precedes citrate which is formed by the transfer of an acetyl group to OAA. OAA is formed by the deamidation of aspartate or condensation of CO2 with pyruvate or PEP. Since mammals do not possess the enzymatic machinery to form TCA cycle intermediates from acetyl CoA, OAA is one of the anaplerotic entry points via pyruvate and pyruvate carboxykinase. MyBioSource\'s Oxaloacetate Assay Kit provides a simple, sensitive and rapid means of quantifying OAA in a variety of samples. In the assay, OAA is converted to pyruvate which is utilized to convert a nearly colorless probe to an intensely colored (lambdamax= 570nm) and fluorescent (Ex/Em = 535/587nm) product. The Oxaloacetate Assay Kit can detect 0.1-10nmol (2-200 uM) of OAA.
Summary: Detection method- Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm)
Species reactivity- Mammalian
Applications- The Oxaloacetate Assay Kit can detect 0.1-10nmol (2-200 uM) of OAA.
Samples: Cell and tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids. Features & Benefits: Simple procedure; takes ~ 40 minutes
Fast and convenient
Kit contains all necessary reagents for accurate measurement of OAA levels.