|The Human OT oxt (Catalog #MBS281516) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS281516 ELISA Kit recognizes Human (General) OT.
The OT oxt product has the following accession number(s) (GI #300675592) (NCBI Accession #ADK26458.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
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Intended Uses: For the quantitative detection of Human Oxytocin (OT) concentration in serum, plasma and other biological fluids.
Principle of the Assay: This assay employs a two-site sandwich enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to OT. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated antibody preparation specific for OT and incubated. Then substrate solution An and B are added to each well. Only those wells that contain OT, HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of OT in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Samples: serum, plasma and other biological fluids
Detection Range: 8 uIU/mL - 400 uIU/mL
Sensitivity: 4 uIU/mL (mean of 6 independent assays). Intra-assay Precision: Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Intra-Assay: CV<8%
Inter-assay Precision: Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV(%) = SD/meanX100. Inter-Assay: CV<10%. Human Oxytocin (OT) ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.