OPN elisa kit product blog
Tags: ELISA Kit; Human ELISA Kit; Osteopontin; OPN; OPN elisa kit;
The Human OPN spp1 (Catalog #MBS037196) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS037196 ELISA Kit recognizes Human OPN.The OPN spp1 product has the following accession number(s) (GI #189405) (NCBI Accession #AAA59974.1) (Uniprot Accession #P10451). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Introduction: ELISA is a simple and highly sensitive method of analysis that allows for simultaneous and rapid quantification of a large number of samples. The assay is based on the specific recognition of the target compound (analyte/antigen) by antibodies which bind to the compound. The antigen-antibody complex is detected and measured with the aid of an enzyme-labeled antibody or antigen. Upon addition of a non-colored reagent, the enzyme produces a color reaction where the color intensity is directly or inversely proportional to the concentration of the analyte in the sample.
Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids
Detection Range: 15.6 - 500 ng/ml.
Sensitivity: 2.0 ng/ml. Intended Uses: This ELISA kit is intended for laboratory in vitro research use only, not for drug, household or other use! The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm by a spectrophotometer. In order to measure the concentration of Human OPN in the sample, this ELISA kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus Human OPN concentration. The concentration of Human OPN in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Intra-assay Precision: Intra-assay CV (%) are less than 15%.
Inter-assay Precision: Inter-assay CV (%) are less than 15%. Human Osteopontin ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. The following patways have been known to be associated with this gene. Blood, Bone, Bone Marrow, Connective Tissue, Heart, Kidney, Liver, Mouth, Muscle, Vascular tissues are correlated with this protein. OPN also interacts with the following gene(s): BMP2, CD44, FN1, ITGA5, ITGAV, ITGB1, ITGB3, PTH, RUNX2. Adenocarcinoma, Breast Neoplasms, Calcinosis, Cardiovascular Diseases, Fibrosis, Heart Diseases, Inflammation, Kidney Diseases, Necrosis, Neoplasms are some of the diseases may be linked to Human Osteopontin ELISA Kit.