OB diy elisa kit product blog
Tags: Obestatin (OB); DIY ELISA Kit; Human DIY ELISA Kit; OB diy elisa kit;
The Human OB lep (Catalog #MBS2089241) is a DIY ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS2089241 DIY ELISA Kit recognizes Human OB.Main materials for "Do It Yourself ELISA Kit". Researchers should empirically determine the suitability of the OB lep for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The OB lep product has the following accession number(s) (GI #623332) (NCBI Accession #AAA60470.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Assay Type: Competitive Inhibition ELISA for Antigen Detection
Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Detection Range: 61.7-5,000pg/mL
Sensitivity: 25.1pg/mL. Reagent Contents: Capture Antibody, Biontin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
Product Usage: 1. Coat the plates with 100muL per well of working solution of Capture Antibody.incubate overnight at 4 degree C or incubate at 37 degree C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 muL per well of working solution of Blocking Buffer. Incubate at 37 degree C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Other Reagents Required: ELISA/CLIA Support Pack 2. (Catalog MBS2089472). OB also interacts with the following gene(s): CRP, GHRL, INS, JAK2, LEPR, PTPN1, SOCS3, STAT3. The following patways have been known to be associated with this gene. Adipose Tissue, Blood, Connective Tissue, Intestine, Mammary Gland, Mouth, Muscle, Placenta, Thymus tissues are correlated with this protein. Cardiovascular Diseases, Fatty Liver, Hyperinsulinism, Inflammation, Insulin Resistance, Neoplasms, Obesity, Pain, Weight Gain, Weight Loss are some of the diseases may be linked to Human Obestatin (OB) ELISA Kit DIY Materials.