NTRK1 elisa kit product blog
Tags: ELISA Kit; Rat ELISA Kit; TrkA; NTRK1 elisa kit; NTRK1;
The Rat NTRK1 ntrk1 (Catalog #MBS175980) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS175980 ELISA Kit recognizes Rat NTRK1.The NTRK1 ntrk1 product has the following accession number(s) (GI #549122) (NCBI Accession #P35739.1) (Uniprot Accession #P35739). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Rat TrkA PicoKine ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only) (Rat TrkA PicoKine ELISA Kit standard curve )
Principle of the assay: rat TrkA ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TrkA has been precoated onto 96-well plates. Standards(NSO, A33-P418) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TrkA is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat TrkA amount of sample captured in plate.
Background: Tropomyosin receptor kinase A (TrkA) are efficacious in attenuating skeletal pain.1 TrkA mutants were able toactivate signaling cascades and were even more efficient in promoting neurite outgrowth than the wild-type receptor.2 TrkA is part of a sub-family of protein kinases which includes TrkB and TrkC. Also, there are otherneurotrophic factors structurally related to NGF: BDNF (for Brain-Derived Neurotrophic Factor), NT-3 (for Neurotrophin-3) and NT-4 (for Neurotrophin-4). While TrkA mediates the effects of NGF, TrkB is bound and activated by BDNF, NT-4, and NT-3. Further, TrkC binds and is activated by NT-3.3 TrkA receptor was found inkeratoconus-affected corneas, along with an increased level of repressor isoform of Sp3 transcription factor.4 Thestandard product used in this kit is extracellular part (A33-P418) of recombinant rat TRKA, consisting of 386 amino acids with the molecular weight of 69KDa.
Samples: Cell culture supernates, Cell lysates and tissue homogenates
Assay Type: Sandwich
Detection Range: 156pg/ml-10,000pg/ml
Sensitivity: < 10pg/ml. Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. Rat TrkA PicoKine ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.