NT-3 elisa kit product blog
Tags: ELISA Kit; Rat ELISA Kit; NT-3 elisa kit; NT-3;
The Rat NT-3 ntf3 (Catalog #MBS355237) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS355237 ELISA Kit recognizes Rat NT-3. MyBioSource\'s NT-3 can be used in a range of immunoassay formats including, but not limited to, Quantitative sELISA (EIA).For quantitative detection of NT-3 in rat serum, body fluids, tissue lysates or cell culture supernatants. Researchers should empirically determine the suitability of the NT-3 ntf3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The NT-3 ntf3 product has the following accession number(s) (GI #156630995) (NCBI Accession #NP_001096124.1) (Uniprot Accession #P20783). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the NT-3 Rat ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-NT-3 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-NT-3 polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the NT-3 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of NT-3 can be calculated.
Background: Neurotrophin-3 is a neurotrophic factor in the NGF (Nerve Growth Factor) family of neurotrophins. It is encoded by the NTF3 gene, which localized to 12p13. It is a protein growth factor which has activity on certain neurons of the peripheral and central nervous system; it helps to support the survival and differentiation of existing neurons, and encourages the growth and differentiation of new neurons and synapses. Ramer et al. (2000) concluded that neurotrophic factor treatment may serve as a viable treatment in promoting recovery from root avulsion injuries. Although the vast majority of neurons in the mammalian brain are formed prenatally, parts of the adult brain retain the ability to grow new neurons from neural stem cells; a process known as neurogenesis.
Assay Type: Sandwich
Detection Range: 15.6 pg/ml-1000 pg/ml
Sensitivity: < 2pg/ml.